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KMID : 0624620090420040223
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BMB Reports
2009 Volume.42 No. 4 p.223 ~ p.226
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pH-Dependent surface-enhanced resonance Raman scattering of yeast iso-1-cytochrome c adsorbed on silver nanoparticle surfaces under denaturing conditions at pH ＜ 3
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Lee So-Yeong
Joo Sang-Woo
Lee Seong-Hoon
Lim Man-Ho
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Abstract
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We measured the pH-induced spectral changes of yeast iso-1-cytochrome c on silver nanoparticle surfaces using surface-enhanced resonance Raman scattering (SERRS) at 457.9 nm. At a pH of ~3, the Met80 ligand in yeast iso-1-cytochrome c is assumed to dissociate, leading to a marked conformational change as evidenced by the vibrational spectral shifts. The Soret band at ~410 nm in the UV-Vis spectrum shifted to ~396 nm at pH~3, indicating a transition from a low spin state to a high spin state from a weak interaction with a water molecule. Thus, SERRS spectroscopy can measure the pH-induced denaturalization of cyt c adsorbed on metal nanoparticle surfaces at a lower concentration with a better sensitivity than ordinary resonance Raman spectroscopy.
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KEYWORD
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Ag nanoparticles, pH change, SERRS, UV-Vis absorption, Yeast iso-1-cytochrome c
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